Recombinant Protein A
CAS:91932-65-9
Non-antibody column purification, no antibody contamination
1. Alias
Staphylococcus aureus protein A (referred to as recombinant protein A or rSPA)
2. Source
Recombinant protein A was expressed in recombinant E. coli and purified by HPLC.
3. Product features and advantages
Form： Lyophilized powder
Ingredients： The recombinant protein A aqueous solution is directly freeze-dried without any other additives.
Purity： (SDS-PAGE detection) >95%
Nucleic acid (UV detection)： DNA, RNA cannot be detected
Triton content： No Triton pollution
Store： at 2℃~8℃
pH stable range： 1-13
1）. Purification by non-antibody column, without any contamination of other antibodies such as IgG in the product.
2）. SPA-25 is a product independently developed by the company. It has a wide range of pH tolerance, can tolerate 0.5 M NaOH and 0.5 M HCl treatment, does not degrade, and the antibody binding ability remains unchanged.
3）. The product has a connecting group specially designed for antibody purification column, which can be directly used for the preparation of protein A column.
4）. The company's recombinant protein A products have the advantages of large-scale production, stable quality and high purity.
4. Product use
Recombinant protein A can be coupled to a solid separation medium (such as agarose) for the purification of monoclonal antibodies or polyclonal antibodies. Recombinant protein A can be coupled with a variety of reporter molecules (fluorescent dyes, enzyme labels, biotin, colloidal gold, radioactive labels, etc.) without affecting its binding activity to antibodies. These coupled protein A derivatives can be used in Western-blot, ELISA, immunohistochemistry and other antibody detection.
5. Product Information
Product name: Recombinant Protein A
Product number: KK-T005
Packaging: 100mg, 1g，10g，100g tube or bottle etc
Product No:KK-T005