Recombinant Trypsin (Porcine)
CAS: 9002-07-7
EC:3.4.21.4
Genetic engineering production, no animal origin, no virus pollution, no miscellaneous enzyme pollution
1. Product introduction
Trypsin is a serine protease that cleaves peptide bonds at the C-terminus of lysine and arginine. Trypsin is widely used in various biotechnological processes. Such as: protein enzymatic hydrolysis to produce polypeptides; cell separation of various tissues; protein enzymatic hydrolysis and sequencing; recombinant insulin production and so on. Recombinant porcine trypsin is expressed and produced by recombinant E. coli, and its amino acid sequence is exactly the same as cationic trypsin derived from porcine pancreas. The specificity of enzyme digestion is the same. The molecular weight is 24kD, and the optimum pH is 7-10. The activity is inhibited by serine protease inhibitors such as PMSF, and metal ion chelating agents such as EDTA inhibit enzymatic activity.
2. Product advantages
No animal origin: recombinant production, no foreign virus contamination, such as swine flu virus, porcine parvovirus, etc. High purity: no other miscellaneous enzyme pollution: no other side reactions in the digestion reaction High specific activity: no less than 3800 USP u/mg pro. Stable: freeze-dried powder, easy to store and transport; mass production, stable product quality
3.Product use
Recombinant porcine trypsin has the same enzymatic properties as animal-derived porcine trypsin, and can replace porcine pancreas-derived trypsin in various biotechnological processes, such as: recombinant insulin production; cell culture, cell fermentation; protein enzymatic hydrolysis , Sequencing; cell separation of various tissues, etc.
Source： Recombinant Escherichia coli
Purification： HPLC
Product properties： white or almost white freeze-dried powder
Electrophoresis identification and Coomassie brilliant blue staining 12% SDS-PAGE： non-reducing SDS-PAGE protein electrophoresis, molecular weight 24kD, single main band
Protein content： 35%～70%
Other enzyme content： No chymotrypsin, carboxypeptidase A and other pollution Does not contain any protease inhibitors： No PMSF, EDTA and other protease inhibitors
4. Product features
Figure 1. SDS-PAGE identification of recombinant porcine pancreatin rpT freeze-dried powder
5. Product Information
Product name：Recombinant trypsin (porcine)
Specific activity: RPT0201 ≥3800 USP u/mg pro.
Packaging:100mg,1g，10g，100g tube or bag or bottle etc
Vitality unit: 25℃, pH7.6, reaction system 3.0ml (1cm light path), each minute of enzymatic hydrolysis of BAEE increases the absorption value at 253nm by 0.003, which is defined as a USP unit.
Product number:KK-T001